Journal: Frontiers in Immunology

Article Title: FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms

doi: 10.3389/fimmu.2024.1467415

Figure Lengend Snippet: Key resources.

Article Snippet: Armenian Hamster anti-mouse CD28 (clone PV-1) , BioXCell , BE0015-5.

Techniques: Recombinant, Fractionation, Cell Culture

Journal: EMBO Reports

Article Title: Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis

doi: 10.1038/s44319-024-00149-y

Figure Lengend Snippet: ( A – C ) MKK3/6 CD4-KO and CD4-Cre mice were fed a high-fat diet (HFD) for 8 weeks. ( A , B ) qRT-PCR analysis of mRNA expression in ( A ) eWAT and ( B ) SVF isolated from control or MKK3/6 CD4-KO mice. mRNA expression was normalized to b-actin mRNA. ( C ) FACS quantification and representative dot plots of IL-35 + Treg cells in lymph nodes. ( D ) In vitro Treg cell induction (iTreg). Naive CD4 + T cells were isolated from the spleens of CD4-Cre and MKK3/6 CD4-KO mice stimulated for 96 h with plate-bound anti-CD3, soluble anti-CD28 + IL-2 + TGFβ. qRT-PCR analysis of p35 and Ebi3 mRNA in iTregs derived from control or MKK3/6 CD4-KO mice. mRNA expression was normalized to b-actin mRNA. ( E ) Induction of iTregs from CD4 + T cells isolated from healthy human donor buffy coats and stimulated with plate-bound anti-CD3, soluble hIL-2 + hTGFβ for 6 days in the presence or absence of the p38 pan inhibitor BIRB796. qRT-PCR analysis of P35 and EBI3 mRNA in iTregs. mRNA expression was normalized to GAPDH mRNA. ( F ) FACS analysis of IL-35 MFI in in vitro induced iTreg cells from CD4-Cre and TSC1 CD4-KO mice. ( G ) Western blot analysis of p-s6 protein S240/244 and p-p38 Thr180/Tyr182 in iTreg cells from CD4-Cre and MKK3/6 CD4-KO mice. Loading control for p-p38 was run on different gel and not presented. ( H ) FACS analysis of IL-35 MFI in in vitro induced iTreg cells from MKK3/6 CD4-KO mice in the presence or absence of rapamycin for 4 h. Data Information: Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant. Exact p-values are shown. Analysis by t test. n = 4–10 biologically independent mice ( A – C ) or n = 4–9 biologically independent wells ( D – H ) for each group, represented as single dots in the graphs. .

Article Snippet: Purified NA/LE Hamster Anti-Mouse CD28 (clon 37.51) , BD Bioscience , Cat# 553294.

Techniques: Quantitative RT-PCR, Expressing, Isolation, In Vitro, Derivative Assay, Western Blot

Journal: EMBO Reports

Article Title: Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis

doi: 10.1038/s44319-024-00149-y

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Purified NA/LE Hamster Anti-Mouse CD28 (clon 37.51) , BD Bioscience , Cat# 553294.

Techniques: Recombinant, Sequencing, SYBR Green Assay, Marker, Purification, Western Blot, Software, Staining, Cell Isolation, Reverse Transcription, Transgenic Assay, Membrane, Electrophoresis, Microscopy